San Francisco, California, United States
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About

Investor & advisor in early stage companies

Experience & Education

  • Accel

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Publications

  • Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen

    PLOS ONE

    The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through…

    The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through fluorescence-activated cell sorting (FACS) analysis, as well as in conditioned medium in a secreted version for function analysis.

    Other authors
    See publication
  • Four-way ligation for construction of a mammalian cell-based full-length antibody display library

    Acta Biochimica et Biophysica Sinica

    A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one step by four-way ligation, and full-length bivalent antibodies were displayed on mammalian cell…

    A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one step by four-way ligation, and full-length bivalent antibodies were displayed on mammalian cell surfaces. Using this strategy, only 2 weeks were required to successfully construct high-quality, full-length human antibody libraries.

    Other authors
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Projects

  • Mind Mentor (2013 Health 2.0 Code-a-Thon Winner)

    - Present

    Despite the ready availability of health-tracking applications, few programs provide a fully integrated platform for monitoring both physical and emotional health.

    Leveraging the built-in Validic API and psychiatric diagnostic testing based on the DSM-5, Mind Mentor provides a safe, user-friendly environment for patient-centric health evaluation.

    From the administrative suite, doctors can schedule psychiatric appointments, therapy activities, create medication reminders, and…

    Despite the ready availability of health-tracking applications, few programs provide a fully integrated platform for monitoring both physical and emotional health.

    Leveraging the built-in Validic API and psychiatric diagnostic testing based on the DSM-5, Mind Mentor provides a safe, user-friendly environment for patient-centric health evaluation.

    From the administrative suite, doctors can schedule psychiatric appointments, therapy activities, create medication reminders, and monitor their patients’ health.

    Patients are empowered by continual reaffirmation, staying connected to their doctor, and by witnessing their own emotional growth.

    Other creators
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Honors & Awards

  • Be a Venture Capitalist Case Competition

    -

    First Place

  • Deloitte Consulting Undergraduate Case Competition

    -

    First Place

  • Haas Undergraduate Leadership Award

    -

    Finalist

  • Jack Larson Center for Entrepreneurship

    -

    Jack Larson Fellow
    Scholarship for Excellence in Entrepreneurship

  • Phi Beta Lambda California State Case Competition

    -

    First Place

  • Siemens Competition in Math, Science, Technology

    -

    Semifinalist

Recommendations received

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